mRNA-Seq of Chinese alligator, male skeletal muscle, wild, winter - SRA

SRX10464664: mRNA-Seq of Chinese alligator, male skeletal muscle, wild, winter
1 ILLUMINA (Illumina HiSeq 2500) run: 35.4M spots, 10.6G bases, 4.2Gb downloads

Design: Strand-specific cDNA libraries were generated from 3 mg RNA per sample, using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to the manufacturers instructions. The index-coded samples were clustered using a TruSeq PE Cluster Kit v3-cBot-HS on a cBot Cluster Generation System (Illumina, San Diego, CA). The libraries were sequenced on an Illumina HiSeq 2500 platform, generating 127-bp paired-end reads.

Submitted by: Zhejiang University

Study: Alligator sinensis mRNA transcriptome of multiple tissues in summer and winter

PRJNA556093 • SRP286845 • All experiments • All runs

show Abstracthide Abstract

The Chinese alligator, a freshwater crocodilian endemic to China, goes into hibernation in refuges during winter to save energy and survive the harshness of winter. In this study, we sequenced mRNA transcriptome of Chinese alligator of multiple tissues to comprehensively explore the molecular mechanism underlying hibernation and provide new insights into the conservation of Chinses alligator which are being threatened by climate change.

Sample:

SAMN18103753 • SRS8595230 • All experiments • All runs

Organism: Alligator sinensis

Library:

Name: WM_MUS

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: PAIRED

Runs: 1 run, 35.4M spots, 10.6G bases, 4.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR14090488	35,381,561	10.6G	4.2Gb	2021-03-29

ID:: 13878424

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity